u87mg cell line  (ATCC)

Journal: PeerJ

Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting

doi: 10.7717/peerj.20583

Figure Lengend Snippet: (A) Green fluorescent protein detection in LN229 and U87MG cell lines. (B) The detection of expression level of ASIC2 by shRNA plasmid. (C) Detection of ASIC2 expression level by shRNA plasmid. (D) Detection of ASIC2 expression level by overexpression plasmid. (E) Detection of cell proliferation ability with U87MG cells. (F) Detection of cell proliferation ability of LN229 cells in each group. (G) Detection of cell clone forming ability of U87MG and LN229 cells. (H) Statistical analysis of colony formation in U87MG and LN229 cells. (I) Detection of cell invasion ability of U87MG and LN229 cells. (J) Statistical analysis of the number of U87MG and LN229 cells migrating through the chamber. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01 vs control, # P < 0.05, ## P < 0.01 vs plasmid control.

Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the U87MG cell line was acquired from the American Type Culture Collection (ATCC).

Techniques: Expressing, shRNA, Plasmid Preparation, Over Expression, Control

Journal: PeerJ

Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting

doi: 10.7717/peerj.20583

Figure Lengend Snippet: (A) Detection of cell migration ability of glioma groups. (B) Statistical analysis of the wound closure rate in U87MG cells. (C) Statistical analysis of the wound closure rate in LN229 cells. (D–E) Regulation of cell cycle by ASIC2 on LN229 cells. (F, H) ASIC2 regulates the expression levels of P21, cycline D1 proteins in LN229. (G, I) Statistical analysis of protein expression in LN229 cells after ASIC2 interference and overexpression. (J–K) ASIC2 regulates the expression levels of MMP2, calcineurin and NFAT1 proteins in glioma cells. (L–O) Statistical analysis of protein expression in U87MG and LN229 cells after ASIC2 interference and overexpression. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control, # P < 0.05, ## P < 0.01, ### P < 0.001 vs plasmid control.

Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the U87MG cell line was acquired from the American Type Culture Collection (ATCC).

Techniques: Migration, Expressing, Over Expression, Control, Plasmid Preparation

Journal: npj Imaging

Article Title: Deep tissue optoacoustic monitoring of photothermal treatments in the NIR-II assisted with silica-coated gold nanorods

doi: 10.1038/s44303-025-00134-7

Figure Lengend Snippet: A (i) Mouse injected with U-87 MG glioma cells, (ii) tumor growth over the period of 12 days (iii) after euthanasia, tumor is injected with AuNRs; B Volumetric OA reconstruction of heat distribution during localized tumor heating; C Tumor heating with a CW laser (8 W/cm 2 , 1064 nm) shown at four different time points from both top and lateral perspectives; D Thermal profile of tumor heating for four different intensities of the CW laser and indicating the four sampled time points shown in ( C ); E Tumor ablation with a CW laser (16 W/cm 2 , 1064 nm) shown at two different time points from both top and lateral perspectives; F OA intensity profile within a region containing the AuNR, indicating the two sampled time points shown in ( E ); G Tumor H&E histology post localized ablation (yellow area) showing ablation zone of thermal coagulation necrosis compared to untreated tumor region in the blue area. The figure was partly created using BioRender.

Article Snippet: U-87 MG glioma cells were obtained from CLS Cell Lines Service GmbH, Germany (Catalog Nr: 300367).

Techniques: Injection, Coagulation